Samsung is now left with the option to appeal the verdict in the US Supreme Court or pay the fine.
A US appeals court has rejected a bid by Samsung to toss a 2012 verdict that found the South Korean phone maker guilty of infringing the patents of Apple.
The US Federal Circuit Court of Appeals has refused to rehear the case over whether its smartphones and tablets infringed the patents owned by Apple.
Major tech companies like Google, Facebook and HP were backing Samsung in its appeal against the verdict.
In its original verdict, the court had awarded $1.05bn in damages in 2012, which was later reduced by US District Court Judge Lucy Koh by $450.5m in March 2013.
In November 2013, a court imposed an additional $290.5m in damages against Samsung, increasing the payout to $930m.
Following the refusal by the US appeals court, Samsung is now left with the option to appeal the verdict in the US Supreme Court or pay the fine.
A Samsung spokesperson was quoted by Cnet as saying: "We are disappointed that our request for an en banc hearing of the US Court of Appeals was denied.
"For decades, we have invested heavily in developing revolutionary innovations in the mobile industry and beyond. We are confident that our products do not infringe on Apple’s design patents, and we will continue to take appropriate measures to protect our products and our intellectual property."
The litigation between two mobile phone makers started way back in 2011, when both the companies filed multi-national lawsuits against each other in the US, EU and Asia, alleging infringement of patents.